Flare is an independent oligo thermodynamics engine for TaqMan assays, built on published nearest-neighbor science.
Parameters come from published science. The optional calibrated mode tunes a handful of parameters against observed reference values, with no access to any third-party source code or binaries. See NOTICE.md.
Run Flare on an assay:
uv run python -m flare SENSE ANTISENSE PROBE
Save the comparison figure as PNG:
uv run python -m flare.plots
Run the frozen tests:
uv run python -m pytest tests/ -q
uv run python -m flare TCTAACTAGCACACTAACTAATGTCA AACACTTGTGCGGTAACCTC CAGAATGTGTTAACCTGTCTTCT
oligo len Tm GC% clamp self hairpin
sense 26 56.96 34.62 1 -1.6 -1.0
antisense 20 56.44 50.00 2 -1.5 0.0
probe 23 55.22 39.13 1 -4.3 -1.8
cross sense antisense -5.1
cross sense probe -2.7
cross antisense probe -3.9
Tm is in °C; self-dimer, cross-dimer and hairpin are ΔG in kcal/mol (0.0 means none found below threshold).